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CRISP-RICE: LSU AgCenter leading the effort to improve sustainability and profitability of rice farming.

No summary available.

LSU AgCenter variety and fungicide trials help determine best management practices.

No summary available.

Effect of LSU and ITS genetic markers and reference databases on analyses of fungal communities

The effect of genetic markers and reference databases on analyses of fungal communities were estimated using fungal large subunit (LSU) and internal transcribed spacer (ITS) amplicon datasets in consecutive years of rhizosphere samples from three candidate biofuel crops, corn (Zea mays), switchgrass (Panicum virgatum), and miscanthus (Miscanthus × giganteus). These two marker genes were selected to contrast possible differences in biological conclusions. In addition, two ITS schemes based on two ITS reference databases were used to assess differences due to reference database composition. A taxonomy-supervised method was invoked using the Ribosomal Database Project naïve Bayesian classifier that accesses all three databases. The UNITE classification scheme had the highest number of classified taxa in the raw classification result; however, it also had the highest proportion of unknown taxa (sequences that were classified to “unclassified,” “unidentified,” incertae sedis or, in the case of Warcup, to matches containing two unique names). After removal of these unknown taxa, LSU had the highest classification rate followed by Warcup and UNITE. As expected, the communities resolved using the two ITS databases, based on the same sequences, were relatively more similar than those from the lower-coverage LSU classification scheme. The choice of marker gene or even the same reads with different classification databases revealed different community patterns due to database coverage, e.g., the relative abundance of the most abundant groups changed or were only detected in one or two of the classification schemes, such as for Mortierella, Fusarium, and Phoma. No marked difference in fungal beta-diversity was identified among the three methods. Differentiation between the three biofuel crops and between the drought and normal rainfall years was apparent, regardless of method. Though classification rates, taxonomic conflicts, and coverage differences within the high-abundance fungal groups varied according to classification scheme, there was no overall impact on beta diversity among the three methods.

Environmental identification of arbuscular mycorrhizal fungi using the LSU rDNA gene region: an expanded database and improved pipeline

Arbuscular mycorrhizal fungi (AMF; Glomeromycota) are difficult to culture; therefore, establishing a robust amplicon-based approach to taxa identification is imperative to describe AMF diversity. Further, due to low and biased sampling of AMF taxa, molecular databases do not represent the breadth of AMF diversity, making database matching approaches suboptimal. Therefore, a full description of AMF diversity requires a tool to determine sequence-based placement in the Glomeromycota clade. Nonetheless, commonly used gene regions, including the SSU and ITS, do not enable reliable phylogenetic placement. Here, we present an improved database and pipeline for the phylogenetic determination of AMF using amplicons from the large subunit (LSU) rRNA gene. We improve our database and backbone tree by including additional outgroup sequences. We also improve an existing bioinformatics pipeline by aligning forward and reverse reads separately, using a universal alignment for all tree building, and implementing a BLAST screening prior to tree building to remove non-homologous sequences. Finally, we present a script to extract AMF belonging to 11 major families as well as an amplicon sequencing variant (ASV) version of our pipeline. We test the utility of the pipeline by testing the placement of known AMF, known non-AMF, and Acaulospora sp. spore sequences. This work represents the most comprehensive database and pipeline for phylogenetic placement of AMF LSU amplicon sequences within the Glomeromycota clade.

Dr. Harry Roberts Boyd Professor Emeritus of Geology at LSU.

The article serves as an obituary for Dr. Harry Roberts, a prominent figure in marine and coastal geology who passed away on March 26, 2025, at the age of 85. Dr. Roberts was a Boyd Professor Emeritus at Louisiana State University (LSU) and made significant contributions to the understanding of river delta dynamics, particularly through his research on the Mississippi River Delta. His work not only advanced scientific knowledge but also influenced coastal management practices and sediment management strategies in Louisiana. Remembered for his warmth and dedication, Dr. Roberts leaves behind a legacy that continues to inspire future generations of geoscientists. [Extracted from the article]

Atmospheric boundary layer simulation in a new open-jet facility at LSU: CFD and experimental investigations

Highlights •Turbulence intensity profiles obtained by LES are in a good agreement with theoretical profiles.•LSU open-jet permits testing larger models with less sensitivity to blockage restrictions.•CFD schemes are potential in the design of open-jet simulators.•CFD reduces the effort required to experimentally simulate near ground ABL wind flows.

Research Guides

284

RNR 4051 Wildlife Habitat Management

A course guide for RNR 4051 Wildlife Habitat Management

EMS 1011 Environment and Technology

EMS 1011 Environment and Technology: Perspective on Environmental Problems

EMS 3050 Environmental Regulations and Compliance

Citizen Science

Guide to understanding Citizen Science

Agri 2001: Special Topics in Agriculture

This is a course guide for Agri 2001. Learn about the research process here.

Information Literacy for Agriculture

Use this guide to help understand Information Literacy in Agriculture.

Scholarly Repository

21034

Faculty Senate Resolution 08-03

Louisiana State University and Agricultural & Mechanical College

S99RS SGR No. 8 (Alcohol-Free Stadium)

Edwards,Wood

F21RS SGR No. 19 (1st parking violation)

William Franques,Daniela Sestich

F22RS SGR 5 (Establishment of Graduate Healthcare Committee)

Adam Dohrenwend,Gabriella Boodhoo,Alicia Cerquone,Leah Clendaniel,Blessing Egbejiogu,Terry Geraldsen,Ritu Ghose,Cullen Hodges,Natalie Kaczynski,Soheil Kafiliveyjuyeh