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A short LSU rRNA fragment as a standard marker for integrative taxonomy in calcareous sponges (Porifera: Calcarea)
Calcareous sponges are taxonomically difficult, and their morpho-systematic classification often conflicts with molecular phylogenies. Consequently, species descriptions that rely solely on morphological characters,and taxonomic revisions appear to provide little to no information about phylogenetic affiliations and integrative approaches, combining DNA and morphological data, are applied more frequently. However, a standardized database that combines DNA sequence and morphological specimen information is still missing for calcareous sponges. The mitochondrial cytochrome oxidase subunit 1 gene (COI) is the marker of choice for rapid species identification in many other animal taxa, including demosponges, for which COI sequences and morphological information have been compiled in the sponge barcoding database (). But due to the peculiarities of calcarean mitochondrial genomes, sequencing COI in Calcarea is methodologically challenging. We here propose the use of one more commonly used DNA marker, the C-region of the 28S gene (LSU), as standard barcoding marker for Calcarea, after also considering the internal transcribed spacer (ITS) region for such proposes. Especially in the subclass Calcaronea, we observed severe problems of high intra- and intergenomic variation that impedes pan-calcarean ITS alignments. In contrast, the C-region of LSU provides a short but phylogenetically informative DNA sequence, alignable across both subclasses with the help of a newly developed secondary structure and which also can be used to address exemplary taxonomic questions. With our work, we start to close the gap of Calcarea in the sponge barcoding project () and provide a resource for biodiversity studies and potentially for DNA-guided species identification.
Are there any graduate assistantships available?
Most assistantships would be found on the LSU Handshake website (https://www.lsu.edu/careercenter/students/handshake.php) , though some opportunities are handled directly through the hiring department. It wouldn't hurt to check with a staff member in your graduate program to see if they are aware of assistantships not listed on Handshake. ________________________________________________________________________ More information on Handshake.... How to Access Handshake Admitted Students Undergraduate and Graduate students receive access to Handshake on June 15. At that time, you can log in to Handshake using your myLSU email and password at lsu.joinhandshake.com (https://lsu.joinhandshake.com/) or download the Handshake Jobs & Careers App (download in the Apple App Store (https://apps.apple.com/app/apple-store/id1220620171) or download through Google Play (https://play.google.com/store/apps/details?id=com.joinhandshake.student…) ). If a user experiences a barrier in access to Handshake or content within due to a disability, please contact the LSU Olinde Career Center at career@lsu.edu (mailto:career@lsu.edu) . For information on how to apply to on-campus and off-campus jobs, visit the Student Employment webpage (https://www.lsu.edu/careercenter/studentemployment/students.php) . If you would like to schedule a meeting with our team, or access other career center resources prior to receiving Handshake access, please contact us at career@lsu.edu (mailto:career@lsu.edu) and we are happy to assist you. Graduate Students: Please note, while some graduate assistantships may be posted in Handshake, most opportunities are managed directly through the hiring department. Please contact your graduate program and campus contacts directly to inquire about available assistantships. Alumni Alumni retain free access to Handshake and to most other career center resources, including appointments with the career center team. View the Alumni Resources page to request Handshake access (https://www.lsu.edu/careercenter/students/alumni.php) . Rsum Uploads Please make note that all rsums must be approved by the LSU Olinde Career Center before becoming active in Handshake for applying for jobs or participating in on-campus interviews. Please be prompt in submitting a rsum for activation in Handshake. The career center makes every effort to be timely in the document approval process, but cannot guarantee a turnaround of less than two (2) business days. Fraudulent and Scam Job Postings We work hard to keep fraudulent postings out of Handshake (https://www.lsu.edu/careercenter/students/handshake.php) by using some common red flags typically considered suspicious. While red flags dont automatically remove a job posting, we research the company and posting if suspicion arises before making a decision. You should research suspicious companies or postings, too (or dont apply). The Fraudulent and Scam Job Postings (https://www.lsu.edu/careercenter/about/FraudulentandScamJobPostingsbook…) guide outlines red flags so you, too, can attempt to identify such scam or fraudulent postings. Our position: Never apply for a suspicious job. Questions? Contact career@lsu.edu (mailto:career@lsu.edu) . Answered by: Gabriella Lindsay

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