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Phylogenetic Analysis of Molluscan Mitochondrial LSU rDNA Sequences and Secondary Structures
Mollusks are an extraordinarily diverse group of animals with an estimated 200,000 species, second only to the phylum Arthropoda. We conducted a comparative analysis of complete mitochondrial ribosomal large subunit sequences (LSU) of a chiton, two bivalves, six gastropods, and a cephalopod. In addition, we determined secondary structure models for each of them. Comparative analyses of nucleotide variation revealed substantial length variation among the taxa, with stylommatophoran gastropods possessing the shortest lengths. Phylogenetic analyses of the nucleotide sequence data supported the monophyly of Albinaria, Euhadra herklotsi + Cepaea nemoralis, Stylommatophora, Cerithioidea, and when only transversions are included, the Bivalvia. The phylogenetic limits of the mitochondrial LSU rRNA gene within mollusks appear to be up to 400 million years, although this estimate will have to be tested further with additional taxa. Our most novel finding was the discovery of phylogenetic signal in the secondary structure of rRNA of mollusks. The absence of entire stem/loop structures in Domains II, III, and V can be viewed as three shared derived characters uniting the stylommatophoran gastropods. The absence of the aforementioned stem/loop structure explains much of the observed length variation of the mitochondrial LSU rRNA found within mollusks. The distribution of these unique secondary structure characters within mollusks should be examined.
Evolutionary relationships of the cup-fungus genus Peziza and Pezizaceae inferred from multiple nuclear genes: RPB2, β-tubulin, and LSU rDNA
To provide a robust phylogeny of Pezizaceae, partial sequences from two nuclear protein-coding genes, RPB2 (encoding the second largest subunit of RNA polymerase II) and β-tubulin, were obtained from 69 and 72 specimens, respectively, to analyze with nuclear ribosomal large subunit RNA gene sequences (LSU). The three-gene data set includes 32 species of Peziza, and 27 species from nine additional epigeous and six hypogeous (truffle) pezizaceous genera. Analyses of the combined LSU, RPB2, and β-tubulin data set using parsimony, maximum likelihood, and Bayesian approaches identify 14 fine-scale lineages within Pezizaceae. Species of Peziza occur in eight of the lineages, spread among other genera of the family, confirming the non-monophyly of the genus. Although parsimony analyses of the three-gene data set produced a nearly completely resolved strict consensus tree, with increased confidence, relationships between the lineages are still resolved with mostly weak bootstrap support. Bayesian analyses of the three-gene data, however, show support for several more inclusive clades, mostly congruent with Bayesian analyses of RPB2. No strongly supported incongruence was found among phylogenies derived from the separate LSU, RPB2, and β-tubulin data sets. The RPB2 region appeared to be the most informative single gene region based on resolution and clade support, and accounts for the greatest number of potentially parsimony informative characters within the combined data set, followed by the LSU and the β-tubulin region. The results indicate that third codon positions in β-tubulin are saturated, especially for sites that provide information about the deeper relationships. Nevertheless, almost all phylogenetic signal in β-tubulin is due to third positions changes, with almost no signal in first and second codons, and contribute phylogenetic information at the “fine-scale” level within the Pezizaceae. The Pezizaceae is supported as monophyletic in analyses of the three-gene data set, but its sister-group relationships is not resolved with support. The results advocate the use of RPB2 as a marker for ascomycete phylogenetics at the inter-generic level, whereas the β-tubulin gene appears less useful.

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Who can advertise on LSU Libraries digital screens, and what are the requirements?
LSU departments and units wishing to promote university events and services may submit slides to be posted on Libraries digital screens. LSU Libraries Digital Screens Dimensions: 1080x1920 and 1920x1080 Guidelines for Submitting a Slide Only LSU departments or units can submit slides for the digital screens. Slides may not be submitted by a: - Faculty member (unless on behalf of a department or unit) - Staff member (unless on behalf of department or unit) - Student (unless on behalf of department or unit) - Student Organization - Sorority or Fraternity - Any group or organization not affiliated with LSU - Businesses Slides must be for official university events and services. All slides must contain the name of the department or unit responsible for the event or service and the ad design must adhere to LSU branding policies. Slides will run for up to two weeks at a time, beginning and ending on weekdays only. Units may request to have no more than two concurrent slides running at any given time. In more can be accommodated, preference will be given to requests submitted first. Submitting a Slide Please submit requests at least five days before you want the slide to be posted. Submit your slide via email to libraries@lsu.edu (mailto:libraries@lsu.edu) . In your email you must include: - Image(s) for the slide. Acceptable file types are .jpg or .png. - Start date to post the slide. - End date if the slide should be removed before the designated two-week period. Slides will be approved if the event is of general importance to LSU staff, students, and/or faculty and meets all other requirements. Answered by: Access Services Staff

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