Forensic analysts often receive information from a multitude of sources. Empirical work clearly demonstrates that biasing information can affect analysts' decisions, and that the order in which task-relevant information is received impacts human cognition and decision-making. Linear Sequential Unmasking (LSU; Dror et al., 2015) and LSU-Expanded (LSU-E; Dror & Kukucka, 2021) are examples of research-based procedural frameworks to guide laboratories' and analysts' consideration and evaluation of case information. These frameworks identify parameters-such as objectivity, relevance, and biasing power-to prioritize and optimally sequence information for forensic analyses. Moreover, the LSU-E framework can be practically incorporated into any forensic discipline to improve decision quality by increasing the repeatability, reproducibility, and transparency of forensic analysts' decisions, as well as reduce bias. Future implementation of LSU and LSU-E in actual forensic casework can be facilitated by concrete guidance. We present here a practical worksheet designed to bridge the gap between research and practice by facilitating the implementation of LSU-E. (© 2022 The Authors.)

An undescribed species of the dinoflagellate genus Karlodinium J. Larsen (viz. K. armiger sp. nov.) is described from Alfacs Bay (Spain), using light and electron microscopy, pigment composition, and partial large subunit (LSU) rDNA sequence. The new species differs from the type species of Karlodinium ( K. micrum (Leadbeater et Dodge) J. Larsen) by lacking rows of amphiesmal plugs, a feature presently considered to be a characteristic of Karlodinium. In K. armiger, an outer membrane is underlain by a complex system of cisternae and vacuoles. The pigment profile of K. armiger revealed the presence of chlorophylls a and c, with fucoxanthin as the major carotenoid. Phylogenetic analysis confirmed K. armiger to be related to other species of Karlodinium; thus forming a monophyletic genus, which, in the LSU tree, occupies a sister group position to Takayama de Salas, Bolch, Botes et Hallegraeff. The culture used by Ballantine to describe Gymnodinium veneficum Ballantine (Plymouth 103) was examined by light and electron microscopy and by partial LSU rDNA. Ultrastructurally, it proved identical to K. micrum (cultures Plymouth 207 and K. Tangen KT-77D, the latter also known as K-0522), and in LSU sequence, differed in only 0.3% of 1438 bp. We consider the two taxa to belong to the same species. This necessitates a change of name for the most widely found species, K. micrum, to K. veneficum. The three genera Karlodinium, Takayama, and Karenia constitute a separate evolutionary lineage, for which the new family Kareniaceae fam. nov. is suggested. [ABSTRACT FROM AUTHOR]

Harmful algal blooms (HABs) caused by an unknown dinoflagellate species have frequently occurred in the Pearl River Estuary, China Since 2006. These blooms were associated with severe water discoloration and economic losses, ranging from several km2 to 300 km2 with the maximum recorded cell density being 2.77 × 107 cells⋅L-1. This unknown dinoflagellate species was initially identified as Cochlodinium geminatum and subsequently reclassified as Polykrikos geminatus. However, after reviewing the original descriptions for Cochlodinium geminatum sensu Schütt (1895) and the genus Polykrikos, we considered this species is incongruent with their original descriptions. Further morphological examinations and particularly phylogenetic analyses based on the SSU and partial LSU rRNA genes of isolates and resting cysts from China and Japan prompted us to consider it a new species of a new genus. This new species was proposed to be Pseudocochlodinium profundisulcus gen. et sp. nov., based on its open comma-shaped apical structure complex (ASC), cingulum encircling the cell less than one and a half turns, a deep sulcus with a torsion of a half turn, either single cell or cell chain consisting of two cells with the same number of nuclei and zooids, the resting cyst bearing lobed ornaments, and the evolutionary distances from Polykrikos (and others) on the phylogenetic trees constructed using the concatenated SSU and partial LSU rRNA gene sequences. Metabarcoding investigation of surface sediment samples collected in China revealed that the species to be widely present along the entire Chinese coast with the highest abundance in the South China Sea. Further re-analysis of the Tara Oceans metabarcoding dataset targeting the SSU rRNA gene V9 domain suggested a global distribution of this new genus. Phylogenetic analyses on 46 OTUs (average length: ∼552 bases) of its LSU rRNA gene sequences (mainly D1-D2 domains) obtained from surface sediment samples revealed intraspecific genetic diversity of this species. Interestingly, based on the different distributions and the abundance of these OTUs along the coast of China, this species appeared to have expanded its distribution from the South China Sea to the northern Yellow Sea, or preferred a warm water habitat. We consider that the present work improves the taxonomy and provides important insights into the biogeography of Pseudocochlodinium profundisulcus.⋅. (Copyright © 2021 Elsevier B.V. All rights reserved.)

In order to estimate the nucleotide diversity of the four kelp species Laminaria digitata , L. hyperborea , L. saccharina and L. faeroensis , three approaches were used. First we sequenced 1320 base pairs (bp) of nuclear-encoded large subunit (LSU) rDNA, which included some of the most variable domains. These sequences discriminated between L. digitata , L. hyperborea and L. saccharina , but L. faeroensis was identical to L. saccharina . A phylogeny inferred based on 672 bp of the nuclear-encoded non-coding internal transcribed spacer regions (ITS1 and ITS2) rDNA, which are more variable than LSU rDNA, likewise failed to resolve a monophyletic L. saccharina with respect to L. faeroensis . Thirdly, we tested the usefulness of the amplified fragment length polymorphism (AFLP) technique to provide a resolved phylogeny for 43 Laminaria specimens. Laminaria digitata and L. hyperborea were resolved as strongly supported monophyletic groups. A third robust clade consisted of L. saccharina plus L. faeroensis. Within this clade, L. faeroensis samples were grouped with bootstrap support of 80%, but the L. saccharina clade was less well supported. The AFLP data in combination with rDNA sequences indicated subspecies status for L. faeroensis . AFLP analyses of different morphotypes of L. digitata and L. saccharina from Danish coastal waters did not reveal any differences. However, L. digitata from the Faroe Islands was genetically distinct from other samples. [ABSTRACT FROM AUTHOR]

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Byline: John Leuzzi, USA TODAY NETWORK It's rivalry week for South Carolina women's basketball, and coach Dawn Staley knows it. The second-ranked Gamecocks will take on No. 4 LSU at […]