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Bento searches all of the available resources at LSU Libraries. Please note that while Discovery does include Catalog results, the dedicated Catalog search can still be accessed.

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Comparison of two short DNA barcoding loci (COI and COII) and two longer ribosomal DNA genes (SSU & LSU rRNA) for specimen identification among quarantine root-knot nematodes (Meloidogyne spp.) and their close relatives
Root-knot nematodes (Meloidogyne spp.) are important pests of numerous crops worldwide. Some members of this genus have a quarantine status, and accurate species identification is required to prevent further spreading. DNA barcoding is a method for organism identification in non-complex DNA backgrounds based on informative motifs in short DNA stretches (≈600 bp). As part of the EU 7th Framework project QBOL, 15 Meloidogyne species were chosen to compare the resolutions offered by two typical DNA barcoding loci, COI and COII, with the distinguishing signals produced by two ribosomal DNA genes (small and large subunit rDNA; SSU ≈ 1,700 and LSU ≈ 3,400 bp). None of the four markers distinguished between the tropical species Meloidogyne incognita, M. javanica and M. arenaria. Taking P ID (Liberal) values ≥0.93 as a measure for species delimitation, the four mtDNA and rDNA markers performed well for the tropical Meloidogyne species complex, M. enterolobii, M. hapla, and M. maritima. Within cluster III A (Holterman et al. Phytopathology, 99, 227–235, 2009), SSU rDNA did not offer resolution at species level. Both mtDNA loci COI and COII did, whereas for LSU rDNA a longer fragment (≥700 bp) is required. The high level of mitochondrial heteroplasmy recently reported for M. chitwoodi (Humphreys-Pereira and Elling Nematology, 15, 315–327, 2013) was not found in the populations under investigation, suggesting this could be a regional phenomenon. For identification of RKNs, we suggest the combined use of SSU rDNA with one of three other markers presented here.
LSU rDNA based RFLP assays for the routine identification of Gambierdiscus species
The Gambierdiscus genus is a group of benthic dinoflagellates commonly associated with ciguatera fish poisoning (CFP), which is generally found in tropical or sub-tropical regions around the world. Morphologically similar species within the genus can vary in toxicity; however, species identifications are difficult or sometimes impossible using light microscopy. DNA sequencing of ribosomal RNA genes (rDNA) is thus often used to identify and describe Gambierdiscus species and ribotypes, but the expense and time can be prohibitive for routine culture screening and/or large-scale monitoring programs. This study describes a restriction fragment length polymorphism (RFLP) typing method based on analysis of the large subunit rDNA that can successfully identify at least nine of the described Gambierdiscus species and two Fukuyoa species. The software programs DNAMAN 6.0 and Restriction Enzyme Picker were used to identify a set of restriction enzymes (SpeI, HpyCH4IV, and TaqαI) capable of distinguishing most of the known Gambierdiscus species for which DNA sequences were available. This assay was tested using in silico analysis and cultured isolates, and species identifications of isolates assigned by RFLP typing were confirmed by DNA sequencing. To verify the assay and assess intra-specific heterogeneity in RFLP patterns, identifications of 63 Gambierdiscus isolates comprising ten Gambierdiscus species, one ribotype, and two Fukuyoa species were confirmed using RFLP typing, and this method was subsequently employed in the routine identification of isolates collected from the Caribbean Sea. The RFLP assay presented here reduces the time and cost associated with morphological identification via scanning electron microscopy and/or DNA sequencing, and provides a phylogenetically sensitive method for routine Gambierdiscus species assignment.

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Why does my library account say that I am blocked, that I am barred, or that my status is expired?
Users can encounter several different types of status messages. Patrons with questions about their account status can visit the checkout desk in room 241 of LSU Library and ask to speak to a staff member. Alternatively, patrons can reach out to us via e-mail at libcirc@lsu.edu (mailto:libcirc@lsu.edu) . When contacting us via e-mail, LSU students, staff, and faculty should message us from their LSU e-mail address; public patrons should message us from the e-mail address we have on file. For privacy reasons, we cannot discuss the details of patron accounts over the telephone. Expired: Students must be currently enrolled in classes in order to be granted library privileges. Once they graduate, or if they fail to register on time in accord with the deadlines posted on LSUs academic calendar, their privileges expire. If they try to log in to their library account after that date, they will see an alert message informing them that their account has expired. Graduate students who have received a masters degree but are continuing on to get their PhD may also have their privileges expire earlier than expected. The library receives weekly updates on student status from the Registrars Office. Once the semester has begun, if students register during the week, their accounts will not be updated and their privileges extended in the system until the following Monday morning. Blocked: Users with overdue recalled books will have their accounts blocked by the system. Their accounts will remain blocked until the book is returned. The system will not permit staff members to override blocks or to renew books that have been recalled. The only way to remove a block from an account is to return the materials. Barred: Users can be barred from using library materials for a number of reasons, the most common being that they have been billed for lost items. They can also be barred if they resign from the university, if their classes are purged, or for flagrant violations of library policy. If they try to log into their account after they have been barred, they will receive an alert message that tells them that they have been barred. Answered by: Access Services Staff

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