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Search all of LSU Libraries in one place. This includes the catalog, research guides, scholarly repository, website, etc.

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Bento searches all of the available resources at LSU Libraries. Please note that while Discovery does include Catalog results, the dedicated Catalog search can still be accessed.

Discovery
Searches our local print and electronic materials including e-books, journal articles, peer-reviewed articles, news, and magazines.
Research Guides
Searches the full-text of research guides published by LSU Libraries. A research guide is a curated, librarian‑built document that pulls together the most important resources for a topic, course, or assignment. It’s designed to help students, faculty, and researchers quickly find high‑quality, relevant information without having to sift through everything on their own.
Scholarly Repository
Searches the full-text of the Scholarly Repository. The LSU Scholarly Repository collects, preserves, publishes, and makes openly accessible the research and scholarship contributed by LSU faculty, staff, students, and units. Research and scholarly archived materials can include articles, monographs, books, theses & dissertations, audio-visual presentations, working papers, technical reports, conference proceedings, special collections, data, and publicly funded research.
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Detecting a Complex of Cryptic Species within Neoechinorhynchus golvani (Acanthocephala: Neoechinorhynchidae) Inferred from ITSs and LSU rDNA Gene Sequences
Neoechinorhynchus golvani is an intestinal parasite of freshwater and brackish water fishes distributed in Mexico. The genetic variability of 40 samples representing 12 populations from north, south, and central Mexico, and 1 from Costa Rica, was estimated by sequencing 2 nuclear genes (ITS1, 5.8S, ITS2, and LSU rDNA, including the domain D2 + D3). The length of both genes ranged from 700 to 779 base pairs (bp) and from 813 to 821 bp, for ITSs and LSU, respectively. The genetic divergence among populations ranged from 19.5 to 35.3% with ITSs and from 9.28 to 19.58% with LSU. Maximum likelihood and maximum parsimony analyses were performed for each data set and also for 2 combined data sets (ITSs + LSU rDNA with and without outgroups), showing strong similarities among trees, with high bootstrap support in all cases. Genetic divergence, in combination with phylogenetic analyses, suggested that the acanthocephalan N. golvani represents a complex of cryptic species, which is composed of at least 3 lineages. The first lineage, corresponding with N. golvani, shows a wide distribution, including localities from northeastern Mexico, southwards through central and southeastern Mexico, and further down to Costa Rica. This lineage is associated with cichlid fishes in strictly freshwater environments. Lineages 2 and 3 are distributed in brackish water systems along the Gulf of Mexico and Pacific slopes, respectively; both are associated with eleotrid fishes, and apparently represent 2 cryptic species. The diversification of the eleotrid and cichlid lineages seems to be the result of independent host-switching events from the ancestral population.
MinION Sequencing of Yeast Mock Communities To Assess the Effect of Databases and ITS-LSU Markers on the Reliability of Metabarcoding Analysis
ABSTRACT Microbial communities play key roles both for humans and the environment. They are involved in ecosystem functions, maintaining their stability, and provide important services, such as carbon cycle and nitrogen cycle. Acting both as symbionts and as pathogens, description of the structure and composition of these communities is important. Metabarcoding uses ribosomal DNA (rDNA) (eukaryotic) or rRNA gene (prokaryotic) sequences for identification of species present in a site and measuring their abundance. This procedure requires several technical steps that could be source of bias producing a distorted view of the real community composition. In this work, we took advantage of an innovative “long-read” next-generation sequencing (NGS) technology (MinION) amplifying the DNA spanning from the internal transcribed spacer (ITS) to large subunit (LSU) that can be read simultaneously in this platform, providing more information than “short-read” systems. The experimental system consisted of six fungal mock communities composed of species present at various relative amounts to mimic natural situations characterized by predominant and low-frequency species. The influence of the sequencing platform (MinION and Illumina MiSeq) and the effect of different reference databases and marker sequences on metagenomic identification of species were evaluated. The results showed that the ITS-based database provided more accurate species identification than LSU. Furthermore, a procedure based on a preliminary identification with standard reference databases followed by the production of custom databases, including only the best outputs of the first step, is proposed. This additional step improved the estimate of species proportion of the mock communities and reduced the number of ghost species not really present in the simulated communities. IMPORTANCE Metagenomic analyses are fundamental in many research areas; therefore, improvement of methods and protocols for the description of microbial communities becomes more and more necessary. Long-read sequencing could be used for reducing biases due to the multicopy nature of rDNA sequences and short-read limitations. However, these novel technologies need to be assessed and standardized with controlled experiments, such as mock communities. The interest behind this work was to evaluate how long reads performed identification and quantification of species mixed in precise proportions and how the choice of database affects such analyses. Development of a pipeline that mitigates the effect of the barcoding sequences and the impact of the reference database on metagenomic analyses can help microbiome studies go one step further.
Does the library offer a book delivery service?
Yes, LSU Library offers book deliveries to faculty and staff only . We will deliver LSU Library books and interlibrary loan books to your departmental mailbox. Reserve and Government Document materials will not be included. To use the service, request the item through your interlibrary loan account. On the request form under "Delivery Options" select "Deliver to Departmental Mailbox." The request will arrive 1-2 business days from the date we receive the item. Heres how to get your materials delivered: - You will need an ILLiad account to make the request. - Once logged in, you will need to select "Deliver to Departmental Mailbox" for your Delivery Method. Books will be delivered to the departmental mailbox of the requestors within 1-2 business days. A second attempt will be made if the delivery is not successful the first time, but if the department office is inaccessible again, patrons will receive an e-mail stating the problem and asking them to come pick up their materials. - The materials will be placed on hold and kept at the checkout desk for 7 days. If they are not picked up in this time frame, they will be returned to the collection. Be aware of the following: - Users are responsible for returning materials back to the library. - Materials will be checked out to the patrons library account prior to being delivered. - The user is responsible for the items requested through this service. - Any LSU Library books not returned or renewed within 40 days of the due date will be considered lost and the usual penalties will apply. - A limit of eight physical material format items can be requested per day. LSU Libraries | Book Delivery Answered by: Access Services Staff

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