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Assessment of the stocks and forecast of the catches of bream (Abramis brama Linnaeus, 1758), common carp (Cyprinus carpio Linnaeus, 1758), roach (Rutilus rutilus Linnaeus, 1758), Prussian carp (Carassius gibelio Bloch, 1782) and pikeperch (Sander lucioperca Linnaeus, 1758) in the Zaporizhzhia (Dnipro) Reservoir for 2025
Purpose. To analyze and summarize the biological parameters of the main commercial species of aquatic bioresources using the example of bream, common carp, roach, Prussian carp and pikeperch of the Zaporizhzhia (Dnipro) Reservoir under conditions of fishery exploitation. Methodology. The study was conducted on multiple-age individuals of of bream, common carp, roach, Prussian carp and pikeperch. Biological analysis of fish was carried out according to classical methods in ichthyology using the following parameters: standard body length, individual weight, sex and condition factor. Biological sampling was carried out during the 2024 growing season during test and commercial fishing in the Zaporizhzhia reservoir in three areas that differed in hydrology and degree of human impact. Findings. Current data on linear-weight parameters of commercial fish species using the example of bream, common carp, roach, Prussian carp, and pikeperch, the age and sex structure of their populations, and fish productivity for the Zaporizhzhia Reservoir have been presented. Scientific and biological justifications, recommendations, and regimes for commercial and bio-reclamation fishing and fish stocking have been developed Originality of the study lies in the comprehensive analysis of biological parameters and the state of commercial fish species of the Zaporizhzhia Reservoir, as well as the development of scientifically based catch limits for 2025. For the first time, modern trends in commercial fishing under martial law conditions have been considered for the reservoir. The study showed changes in the structure of populations and fish productivity of the reservoir, which allowed us to offer recommendations for the rational use of aquatic biological resources and ensuring the stability of the exploitation of ichthyofauna. Practical Value. The Zaporizhzhia Reservoir has the potential for the development of fishing, fish farming and aquaculture in the Prydniprovskyi region. The results of the study showed that it is possible to set limits on the catch of the studied fish species: bream – 250 tons, common carp - 120 tons, roach - 400 tons, Prussian carp - 1500 tons, pikeperch - 50 tons. Based on the conducted study, recommendations were provided on establishing forecasts of catch and limits for the harvest of aquatic biological resources from the Zaporizhzhia Reservoir for 2025.
Redox-Responsive 'Catch and Release' Cryogels: A Versatile Platform for Capture and Release of Proteins and Cells
Macroporous cryogels are attractive scaffolds for biomedical applications, such as biomolecular immobilization, diagnostic sensing, and tissue engineering. In this study, thiol-reactive redox-responsive cryogels with a porous structure are prepared using photopolymerization of a pyridyl disulfide poly­(ethylene glycol) methacrylate (PDS-PEG-MA) monomer. Reactive cryogels are produced using PDS-PEG-MA and hydrophilic poly­(ethylene glycol) methyl ether methacrylate (PEGMEMA) monomers, along with a PEG-based cross-linker and photoinitiator. Functionalization of cryogels using a fluorescent dye via the disulfide-thiol exchange reactions is demonstrated, followed by release under reducing conditions. For ligand-mediated protein immobilization, first, thiol-containing biotin or mannose is conjugated onto the cryogels. Subsequently, fluorescent dye-labeled proteins streptavidin and concanavalin A (ConA) are immobilized via ligand-mediated conjugation. Furthermore, we demonstrate that the mannose-decorated cryogel could capture ConA selectively from a mixture of lectins. The efficiency of protein immobilization could be easily tuned by changing the ratio of the thiol-sensitive moiety in the scaffold. Finally, an integrin-binding cell adhesive peptide is attached to cryogels to achieve successful attachment, and the on-demand detachment of integrin-receptor-rich fibroblast cells is demonstrated. Redox-responsive cryogels can serve as potential scaffolds for a variety of biomedical applications because of their facile synthesis and modification.