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Studies on woloszynskioid dinoflagellates VII. Description of Borghiella andersenii sp. nov.: light and electron microscopy and phylogeny based on LSU rDNA.
Freshwater woloszynskioid dinoflagellates were collected independently in Scotland and Portugal and found to belong to a previously unknown species of the genusBorghiella, here described asB. andersenii. The new species differs in morphology and nuclear-encoded LSU rDNA and ITS sequences fromB. dodgeiandB. tenuissima, the two species presently comprising the genusBorghiella. Unusual features of the new species were observed particularly during asexual reproduction, which took place in the motile stage – as in many other dinoflagellates – or in a so-called division cyst, recalling cell division in the family Tovelliaceae. Such diversity in cell division is rarely reported in dinoflagellates. MorphologicallyBorghiella anderseniidiffers fromB. tenuissimain being only slightly compressed dorsoventrally whereas the latter species is flat. The slight compression is also visible in lateral view.Borghiella anderseniiandB. dodgeiare more challenging to discriminate but the apical structure complex is only half the length inB. anderseniicompared withB. dodgei(3–4 vs 6 µm). This difference can only be accounted for in the scanning electron microscope. At the light microscopy level the epicone inB. anderseniiis rounded whereas it is conical inB. dodgei. Sexual reproduction inBorghiella anderseniiwas homothallic by formation of planozygotes, followed by apparent resting cysts. Phylogenetic studies on woloszynskioids have recently shown that they comprise a polyphyletic assemblage, which has been divided into the three families Borghiellaceae, Tovelliaceae and Suessiaceae. New species of the three families are now being found rapidly in many parts of the world, proving that the techniques required to investigate these small, morphologically similar dinoflagellates are now in place and proving that such ‘gymnodinioids’ or ‘woloszynskioids’ comprise an often overlooked biological entity in both marine and freshwater biotopes. Based on LSU rDNA,B. anderseniiis most closely related toB. tenuissima. [ABSTRACT FROM AUTHOR]

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