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2065115
Guinean landing surveys of Bentfin devil rays add insight into threatened elasmobranch catch
Populations of many elasmobranchs have decreased globally, with over a third of species threatened with an elevated risk of extinction, largely due to fishing pressure. In West Africa, a general lack of species-specific catch data limits the ability to implement effective management, despite significant elasmobranch catch. The aim of this study was to (1) assess landings of mobulid rays, along with other elasmobranch species, recorded opportunistically in fisheries from Guinean waters, (2) provide information on the value and trade of these species, and (3) discuss management implications. Elasmobranchs were photographed and identified during market surveys over a ten-month period in 2014 and 2015, at four local Guinean fish markets: Bonfi, Boulbinet, Kassa, and Kamsar. A total of 1954 specimens, comprising 17 different elasmobranch species, were recorded across all markets. Of these species, 94% are assessed as threatened (vulnerable, endangered, critically endangered) and 6% are assessed as Near Threatened on the IUCN’s Red List of Threatened Species. The only mobulid species recorded was Mobula thurstoni, with significantly larger specimens recorded in Boulbinet, while in Bonfi fishing grounds overlapped with young-of-the-year specimens. When considering all elasmobranch landings, Bonfi and Boulbinet had similar species compositions, although when considering daily data, species composition tended to differ. Indicator Species Analysis indicated that Carcharhinus falciformis, Carcharhinus leucas, and Mustelus mustelus were top indicator species for Bonfi (with high specificity and fidelity), while Paragaleus pectoralis and Gymnura sereti were key indicator species for Boulbinet. Records of selling price at Bonfi were higher for various stingray species than larger shark species, suggesting meat as a primary driver of the fishery. This study adds insight into the species composition of elasmobranch catch in West Africa and highlights specific fishing sectors contributing to elasmobranch exploitation locally; information which is needed to understand the effects on regional stocks.
Effect of J-Hook Shapes on Catch Rate, Efficiency, and Hooking Position of Needlefish : Evidence from Palk Bay, India
The efficiency of different shapes of J hooks No. 11 (nonoffset straight hook, 10° offset kirbed hook, and 10° offset reversed hook) in needlefish longline fishing gears was studied in 24 fishing grounds in Palk Bay, India, from, February to July 2022. A total of 24 fishing trips were conducted for each experimental gear for the comparative study, with 14,400 hooks, with a total catch of 582 fish during the study. The result was that the kirbed hook had the highest percentage composition of the needlefish (37.36%), followed by the reversed hook (31.79%) and the straight hook (30.75%). Further, the overall hooking rate was higher for the kirbed hook than for the straight hook (13.43% vs. 8.60%). Considering the catch rate, the overall catch per unit effort (CPUE; individual/200 hooks) of the kirbed hook was higher (9.08 vs. 7.45) than that of the straight hook, whereas for the straight hook, the CPUE of nontarget species (0.29) was much higher than that of the kirbed hook (0.12). In terms of the targeted species, the Ablennes hians showed the highest CPUE of 3.78 individuals/200 hooks followed by Tylosurus crocodilus crocodilus (1.78), Strongylura strongylura (1.38); the least dominant CPUE was T. choram (0.94). The percentage of hooking position in the jaw was higher in the kirbed hook than that of the straight hook (64.7% vs. 39.1%) and was found lower in the gut system (11.5% vs. 28.5%). Among the three hook shapes tested, the overall performance of the 10° offset kirbed hook was found to be better than the other 10° offset reversed and nonoffset J hook in terms of a higher catch efficiency, hooking rate, CPUE, and hooking position for needlefish (< 0.05).
BE-CATCH: Bioamplifier-Equipped CRISPR-Cas12a Transduction System Coupled with Commercial Pregnancy Test Strips to Harness Signal-on Point-of-Care Detection
Repurposing existing commercial diagnostic equipment to enable portable analysis of diverse targets is driving the development of affordable point-of-care testing (POCT). Interestingly, we found that goat antimouse IgG could replace human chorionic gonadotropin (hCG) to make the T line of pregnancy test strips (PTS) appear red color and accordingly synthesized a novel signal output probe, which eliminated the intricate hCG covalent coupling steps, and could meet the multiple needs of expanded POCT. Given this, we introduced a novel separation-free universal POCT strategy termed bioamplifier-equipped CRISPR-Cas12a transduction system coupled with PTS to harness signal-on detection (BE-CATCH). Specifically, target inputs were converted and amplified by the multiplied strand displacement amplification-based bioamplifier, thereby activating Cas12a’s trans-cleavage activity. Then, the activated Cas12a would cleave the connector indiscriminately, which ultimately kept the signal output probe in a free state; thus, the inputs could be translated into a colorimetric signal on the PTS. This strategy not only provided boosted sensitivity and specificity but also enhanced user-friendliness by maintaining the signal-on detection mode. We also demonstrated the versatility of the BE-CATCH strategy through selectively detecting miR-155 and flap endonuclease 1. Given its broad adaptability, the BE-CATCH strategy could provide an appealing option to broaden the application of PTS in biomedical diagnostics.