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Trust no one. Believe nothing...DI Andy Horton is called out to examine a gruesome catch by two fishermen: a human hand. Is it that of missing violent criminal, Alfie Wright – or is he the killer? And where is the rest of the corpse?Soon Horton finds himself immersed in a complex case where everyone has a reason to lie and no one is who they seem. Assailed by doubts both in his personal and professional life, Horton desperately tries to keep his emotional feelings under control and his focus on his work. His instincts tell him to trust no one and believe nothing; he's not sure, though, whether this time he'll succeed...

Although processors can be a significant source of fishery-based employment and rent generation, land-based processors are often not included in analyses of fishery management impacts, generally because no data are available. This article utilizes facility-level, cost-earnings data on shoreside processors in the Pacific whiting fishery to describe behavior before and after rationalization in 2011, where 20% of the harvesting quota was allocated to processors. Predicted effects on processors centered on the potential for season lengthening to lead to increased ex-vessel prices and efficiency gains in the use of processing inputs, like labor and utilities. Data show longer seasons following rationalization, with processors paying higher ex-vessel prices as the ratio of ex-vessel to export prices fell. Estimated changes in efficiency match predictions directionally, but are not statistically significant, which may be due, in part, to the limited number of facilities and the range of other factors from which the impacts of rationalization must be disentangled.

Focal adhesions (FAs) are large, integrin-based protein complexes that link cells to the extracellular matrix (ECM). FAs form only when and where they are necessary to transmit force between the cellular cytoskeleton and the ECM, but how this occurs remains poorly understood. Talin is a 270-kDa adaptor protein that links integrins to filamentous (F)-actin and recruits additional components during FA assembly in a force-dependent manner. Cell biological and developmental data demonstrate that the third and C-terminal F-actin–binding site (ABS3) of talin is required for normal FA formation. However, purified ABS3 binds F-actin only weakly in solution. We used a single molecule optical trap assay to examine how and whether ABS3 binds F-actin under physiologically relevant mechanical loads. We find that ABS3 forms a catch bond with F-actin when force is applied toward the pointed end of the actin filament, with binding lifetimes >100-fold longer than when force is applied toward the barbed end. Long-lived bonds to F-actin under load require the ABS3 C-terminal dimerization domain, whose cleavage has been reported to regulate FA turnover. Our results support a mechanism in which talin ABS3 preferentially binds to and orients actin filaments with barbed ends facing the cell periphery, thus nucleating long-range order in the actin cytoskeleton. We suggest that talin ABS3 may function as a molecular AND gate that allows FA growth only when sufficient integrin density, F-actin polarization, and mechanical tension are simultaneously present.

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